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Project : Faisabilité du dépistage in vitro de la tuberculose bovine IFN-γ et ELISA Ac dans les conditions de terrain

Duration of the project

  • Starting date : 01/01/2016
  • Ending date : 31/12/2016

Operational Direction

Bacterial Diseases

Operational Service


Role of CODA-CERVA inthis project :



The main objective of this project is to assess the feasibility and performance of an "in vitro" screening (IFN-γ and ELISA Ac) of bovine tuberculosis in field conditions by comparison with the conventional approach based on the intradermal tuberculin test. In more precise terms, this project aims to cover 4 types of objectives:
  • those covering the logistical aspects of the IFN-γ (sampling and routing);
  • those relating to the technical aspects of the tests;
  • those relating to the epidemiological aspects of "in vitro" screening of tuberculosis; and
  • those relating to the financial aspects of in vitro screening.

In logistical terms, the objectives are as follows:
  • Evaluation of the feasibility and costs relating to the collection and routing of the samples to the analysis laboratory.
  • Development of appropriate sampling procedures for the IFN-γ test as part of a winter campaign.
  • Development of practical procedures that guarantee the "hot chain."
  • Collection of operational data to assess the cost of the aspects better.

The objectives in technical terms are as follows:
  • Evaluation of the "robustness" of the IFN-γ test, i.e. obtaining comparable results between laboratories.
  • Determination of the impact of the basal rate of INF-γ obtained on the samples taken under field conditions on the interpretation of the results of the test. Note: The term "basal" rate refers to the rate of IFN-γ present in the blood outside any antigenic stimulation.
  • Comparison of the response of leucocytes to different "synthesis" antigens (ESAT-6 & CFP-10) which are purportedly more specific than the conventional antigens derived from cultures (PPDB and PPDA).

From an epidemiological point of view, the project aims essentially to assess the advantages and to identify the limits of "in vitro" screening by comparison with the conventional approach.
Given the currently favourable epidemiological context of Belgium, this evaluation will pertain essentially to the specific nature of this approach. An evaluation in terms of sensitivity will be possible only if confirmed sources are detected and included in the course of the project.

Finally, from a financial point of view, the objective of this pilot project is to gather a series of operating data that will make it possible to calculate the related costs of this type of screening with precision.



CODA-CERVA scientistresponsible for this project: Virigine Roupie




Internal partners


External partners


Source of funding

Fond Sanitaire