Home Provision of services Electron Microscopy
Electron Microscopy Print E-mail

Diagnostic EM

Routine diagnosis

Electron miscroscopy CODA-CERVAQuality control of biological samples
  • Control of animal cell cultures for contaminating agents includingmycoplasma, fungi, retro- and paramyxoviruses
  • Control of virus stock solutions, antigens and adventitious viruses like "931" simian paramyxovirus
  • Check for the absence of bacteriophages
  • Routine diagnosis of field samples including rota - corona differential diagnosis in faeces samples, RHV calicivirus in liver
  • Confirmatory diagnoses of viral pathogens from both cell culture supernatans and allantoic fluid

Orienting diagnosis of new, (re)emerging or rare pathogens

  • Rapid differential diagnosis FMD - Orbivirus - parapoxvirus
  • Rapid differential diagnosis of zoonotic pox viruses on animal an human samples - See Guidelines

EM-research

Development, introduction and validation of methodologiesElectron microscopy CODA-CERVA
  • In the past: immunogold staining, on-grid ultracentrifugation, pop-off method, microwave-assisted embedding, lectin based glycoprotein detection. These methods are currently used in diagnostic EM and EM-research
  • Cryofixation and cryo-elektronmicroscopy
  • Electron tomography of semi-thin sections
  • Cryo-electron tomography
  • Detection systems for immunogold staining using Primary Ab of various species
  • Semi-automatic detection of (nano)particles

Descriptive electron microscopy

Quantification of particles and antigens on an unity basis by MET

  • Analysis of virus-like particles (VLPs) like feline calicivirus, canine parvovirus, bluetongue virus, AI-H5N1 MMLV, porcine circovirus, human norovirus, bovine calicivirus
  • Phenotypical analysis of viruses and bacteria based on immunogold staining including recombinant herpesviruses, reconminant paramyxoviruses, recombinant poxviruses, Lawsonia intracellularis)

Scientists responsible for electron microscopy at CODA-CERVA

Jan Mast

Pieter-Jan De Temmerman